Increased fertility of Corynebacterium glutamicum recipients in intergeneric matings with Escherichia coli after stress exposure.

نویسندگان

  • A Schäfer
  • J Kalinowski
  • A Pühler
چکیده

Corynebacterial recipient cells exposed to heat, organic solvents, pH shifts, or detergents show an increased fertility in subsequent interspecific matings with Escherichia coli. This effect is independent of de novo protein biosynthesis and seems to be due to a direct inactivation of a restriction system active against foreign DNA that enters the cell by IncP-mediated conjugation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Characterization of OxyR as a Negative Transcriptional Regulator That Represses Catalase Production in Corynebacterium diphtheriae

Corynebacterium diphtheriae and Corynebacterium glutamicum each have one gene (cat) encoding catalase. In-frame Δcat mutants of C. diphtheriae and C. glutamicum were hyper-sensitive to growth inhibition and killing by H(2)O(2). In C. diphtheriae C7(β), both catalase activity and cat transcription decreased ~2-fold during transition from exponential growth to early stationary phase. Prototypic O...

متن کامل

Influence of membrane composition on osmosensing by the betaine carrier BetP from Corynebacterium glutamicum.

The glycine betaine carrier BetP from Corynebacterium glutamicum was recently shown to function as both an osmosensor and osmoregulator in proteoliposomes made from Escherichia coli phospholipids by sensing changes in the internal K+ concentration as a measure of hyperosmotic stress (Rübenhagen, R., Morbach, S., and Krämer, R. (2001) EMBO J. 20, 5412-5420). Furthermore, evidence was provided th...

متن کامل

Engineering of a glycerol utilization pathway for amino acid production by Corynebacterium glutamicum.

The amino acid-producing organism Corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of Escherichia coli glycerol utilization genes, C. glutamicum was engineered to grow on glycerol. While expression of the E. coli genes for glycerol kinase (glpK) and glycerol 3-phosphate dehydrogenase (glpD) was sufficient for gro...

متن کامل

Expression of the Corynebacterium glutamicum panD Gene Encoding L-Aspartate-a-Decarboxylase Leads to Pantothenate Overproduction in Escherichia coli

The Corynebacterium glutamicum panD gene was identified by functional complementation of an Escherichia coli panD mutant strain. Sequence analysis revealed that the coding region of panD comprises 411 bp and specifies a protein of 136 amino acid residues with a deduced molecular mass of 14.1 kDa. A defined C. glutamicum panD mutant completely lacked L-aspartate-a-decarboxylase activity and exhi...

متن کامل

Multicopy suppression by asd gene and osmotic stress-dependent complementation by heterologous proA in proA mutants.

Auxotrophic proA mutants of Escherichia coli were complemented by two different classes of Corynebacterium glutamicum genes. One of these was the asd gene. The E. coli asd gene also complements the same proA alleles. Complementation of proA by the asd+ gene requires a high asd dosage and the proB and the proC gene products. The reciprocal complementation pattern (asd by the proA+ gene) was not ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Applied and environmental microbiology

دوره 60 2  شماره 

صفحات  -

تاریخ انتشار 1994